SLU-PP-332 Metabolic Research Capsule Overview

SLU-PP-332 is a high-purity, research-grade small molecule and “exercise-mimetic” agonist of estrogen-related receptors (ERRs), designed to model mitochondrial biogenesis, fat utilization, and cellular energy regulation in controlled experimental systems. In capsule form, it enables non-invasive, orally delivered investigation of ERR-driven transcriptional programs and metabolic remodeling in vivo.

Molecular Composition and Mechanistic Profile

• SLU-PP-332: A synthetic small molecule originally developed at Saint Louis University, characterized as a potent, non-selective pan-agonist of estrogen-related receptors ERRα, ERRβ, and ERRγ, with highest potency at ERRα.

• Nuclear receptor activity: Cell-based cotransfection/reporter assays report EC50 values of approximately 98 nM for ERRα, 230 nM for ERRβ, and 430 nM for ERRγ, indicating strong engagement of the ERRα isoform central to energy metabolism and mitochondrial regulation.

  Mechanistically, SLU-PP-332:

  Activates ERR–PGC-1α signaling, a master regulatory axis controlling mitochondrial biogenesis, oxidative phosphorylation, and fatty acid oxidation.

• Induces an “acute aerobic exercise”–like gene expression signature, including

  upregulation of stress-response genes such as DDIT4, which links nutrient

  sensing, mTOR signaling, and cellular energetics.
  Enhances mitochondrial function and cellular respiration in skeletal muscle cell

  lines and increases fast oxidative (Type IIa) muscle fiber content and endurance capacity in murine models.

  These properties position SLU-PP-332 as a probe for dissecting exercise-mimetic pathways, lipid handling, and metabolic flexibility.

Integrated Research Applications

• SLU-PP-332 capsules are formulated for oral administration in animal and other preclinical models, enabling chronic ERR activation studies without injections. Key experimental domains include:

• Exercise-mimetic and performance models: Evaluation of run time to exhaustion, VO2 max, capillary density, oxidative fiber shift, and mitochondrial content in otherwise sedentary animals.

• Metabolic health and obesity models: Investigation of diet-induced obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD/NASH), including effects on white adipose tissue mass, hepatic steatosis, fasting insulin and glucose, and systemic inflammatory markers.

• Substrate utilization and metabolic flexibility: Studies of how skeletal muscle, liver, and adipose tissue partition lipids vs. carbohydrates as fuel, including changes in lipid uptake, oxidation, and thermogenesis.

• Cellular and molecular assays: In vitro work on mitochondrial respiration, gene expression profiling of ERR/PGC-1α targets, and assessment of DDIT4-mediated stress signaling and downstream pathways.

  By encapsulating a defined dose per unit, these formulations support standardized chronic-dosing protocols and longitudinal metabolic investigations.

Analytical Validation and Quality Assurance

• SLU-PP-332 research capsules are typically specified as containing 250 mcg of active compound per capsule, with configurations such as 30- or 60-count bottles (e.g., 250 mcg × 60 capsules = 15,000 mcg total). Supplier documentation emphasizes:

  High purity (commonly ≥98–99%) verified through analytical methods such as HPLC.

  Structural identity and molecular weight confirmation via mass spectrometry and related techniques.

  Batch consistency, labeling of total SLU-PP-332 content, and clear research-use designation.

  These controls facilitate reproducible dosing across experiments and maintain quality

expectations similar to other advanced research peptides and small molecules. Storage and Stability Guidelines

• Although SLU-PP-332 is formulated in capsules rather than lyophilized vials, standard small-molecule storage practices still apply. Typical guidance from suppliers and reference labs includes:

• Store capsules in a cool, dry place, protected from light and excessive humidity, ideally at or below room temperature.

• For bulk powder, maintain at approximately −20 °C to preserve chemical stability over extended timelines.

• Keep containers tightly closed and avoid prolonged exposure to high temperatures or moisture to limit degradation.

Adhering to these conditions helps ensure consistent potency and reliability in long-term research programs.

At NextGenPeps, SLU-PP-332 is provided exclusively to qualified investigators for metabolic, transcriptional, and exercise-physiology research in non-human models and cell-based systems. It is not designed, marketed, or allowed for human consumption or performance enhancement, and any use must remain within the boundaries of compliant laboratory research.​